RESEARCH PAPER
EVALUATING THE RELIABILITY OF VASTUS MEDIALIS THICKNESS MEASUREMENT USING B-MODE ULTRASOUND: A ONE-WEEK INTERVAL STUDY
 
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1
Institute of Physiotherapy and Health Sciences, Academy of Physical Education, Katowice, Poland
 
2
Functional Diagnostics Laboratory, Sport-Klinika, Scanmed Sport, Żory, Poland
 
3
Iga Garbowska Osteopathy Clinic, Katowice, Poland
 
4
Department of Immunobiology and Environment Microbiology, Medical University of Gdańsk, Gdańsk, Poland
 
5
McConnell Physiotherapy Group, Mosman, Australia
 
6
Centre for Health Exercise and Sports Medicine, University of Melbourne, Melbourne, Australia
 
 
Submission date: 2025-08-14
 
 
Final revision date: 2025-09-17
 
 
Acceptance date: 2025-09-19
 
 
Publication date: 2025-09-24
 
 
 
KEYWORDS
TOPICS
ABSTRACT
Background:
The vastus medialis (VM) muscle is essential for patellofemoral joint stability, and its dysfunction is linked to multiple knee disorders. Ultrasound (US) offers a practical method for VM assessment. The reliability of longitudinal-plane thickness measurements by novice examiners remains unclear.

Material and methods:
18 healthy adults (mean age 23.5±1.25 years) underwent three VM thickness measurement sessions: two on the same day and one after one week. Measurements at rest (R) and during maximal voluntary isometric contraction (MVIC) were obtained using a standardized protocol. Intra-class correlation coefficients (ICCs), standard error of measurement (SEM), and smallest detectable difference (SDD) were calculated to assess intra-rater reliability.

Results:
Single-session measurements achieved excellent reliability (ICC≥0.93) for both R and MVIC, with greater precision from averaging multiple registrations. One-week interval measurements showed moderate-to-good reliability (ICC 0.52-0.87), improving with repeated measurements. Mean VM thickness increased from 27.1±3.9 mm (R) to 32.4±4.6 mm (MVIC).

Conclusions:
Longitudinal US measurement of VM thickness is highly reliable within a single session, especially when multiple images are averaged. Consistent landmarks, standardized procedures, and examiner training can enhance long-term measurement reliability in clinical and research settings.
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ISSN:2353-6942
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